Latin Square Design

 

Example:

 

A laboratory is interested in examining the leaves of apple trees to detect deficiencies using three different laboratory procedures (is there a difference in mean readings for apple leaves utilizing three different lab. Procedures). Assume we use three analyst for leaf assays (not one). Randomly assign three apple leaves to each analyst.  Suppose that each leaf assay takes a long time and only one procedure can be done by each analyst per day. By modifying the randomized block design as follows

 

Must restrict our randomization to ensure that each treatment appears in each row ( day ) and in each column ( analyst )

 

 

 

 

 

 

                   Analyst

Day

1

2

3

1

A

B

C

2

B

C

A

3

C

A

B

 

 

Latin square design can be used to compare t treatment means in the presence of two extraneous sources of variability, which are blocked off into t rows and t columns. The t treatments are then randomly assigned to the rows and columns so that each treatment appears in every row and every column of the design